# Osmometry Molecular Weight Determination? Trust The Answer

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## Membrane osmometry method to find number average molecular weight of polymer

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## How is molecular weight of polymers determined by membrane osmometry?

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Pharmaceutical and Biomedical Applications of Polymers

Osmometry is used to determine the molecular mass, which depends on colligative properties, meaning that the number of dissolved molecules is the only factor that alters the properties of a solution.

What type of molecular weight of polymer is determined by osmometry?

There are two principal methods of osmometry that are suitable for determining average molecular weights of polymers: membrane and vapor pressure osmometry.

How is the molecular weight of a polymer determined?

Determining Polymer Molecular Weight

Molecular weight is determined by multiplying the mass of each subsequent repeating unit in the polymer chain, before adding the mass of either end group. This provides a range of values that are used to establish the molecular weight distribution of the material.

What molecular weight average is obtained from a membrane osmometer?

The upper limit of molecular weights to which the vapor phase osmometer can be applied is usually considered to be 20,000 g mol1. Newer, more sensitive machines have extended this limit to 50,000 g mol1 or higher.

How will you determine the molecular masses of polymers by osmotic pressure method?

The flow of solvent that occurs due to a concentration gradient across the membrane is called osmosis. According to Equation 5, the molecular weight of a solute can be obtained by plotting osmotic pressure divided by c versus concentration and extrapolating the data back to c=0.

## What is the principle of osmometry?

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Osmometry is an advanced analytical method for determining the osmotic concentration of solutions. The osmotic – or solute – concentration of a colloidal system is expressed in osmoles (Osm) per unit of volume (Osm/L) or weight (Osm/kg).

What is the principle of osmolality?

The urine osmolality determination is based upon the principle that increased concentration of a solute in a solution causes lowering of its freezing point. This method is referred to as freezing point depression osmometry.

What is osmometry used for?

Osmometers are useful for determining the total concentration of dissolved salts and sugars in blood or urine samples. Osmometry is also useful in determining the molecular weight of unknown compounds and polymers. Osmometry is the measurement of the osmotic strength of a substance.

What is the principle of freezing point osmometer?

Freezing point osmometry

The measurement principle of the K-7400S Semi-Micro Osmometer is based on the colligative property of freezing point depression. If a solute is added to a liquid this results in a decreased freezing point of the solution.

What is the principle of measuring osmolality by freezing point depression?

When a solute (particles) is dissolved in a solvent (water), the freezing point of that solution is lower than that of the solvent alone. As more solute is added, the freezing point decreases further. By precisely measuring the freezing point of the solution, the osmolality, or concentration, can be determined.

## How do you find the molecular weight of polymer by osmotic pressure method?

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The flow of solvent that occurs due to a concentration gradient across the membrane is called osmosis. According to Equation 5, the molecular weight of a solute can be obtained by plotting osmotic pressure divided by c versus concentration and extrapolating the data back to c=0.

How do you calculate molecular weight from osmotic pressure?

The flow of solvent that occurs due to a concentration gradient across the membrane is called osmosis. According to Equation 5, the molecular weight of a solute can be obtained by plotting osmotic pressure divided by c versus concentration and extrapolating the data back to c=0.

How the molecular mass is determined by osmotic pressure method in polymers?

<br> (R) : Osmotic pressure can be accurately determined for polymer of high molecular mass as it is a colligative property. Colligative properties can be used to determine the molecular mass of solutes in solutions. For determining the molecular mass of polymers, osmotic pressure is preffered to other properties.

How do you find the molecular weight of a polymer?

Therefore, in order to determine the molecular weight and molecular weight distribution of a polymer, one of the most widely applied methods is gel-permeation chromatography. Gel permeation chromatography (GPC) is a term used for when the separation technique size exclusion chromatography (SEC) is applied to polymers.

How do you calculate the osmotic pressure of a polymer?

Π / c ≈ RT Mn1 · {1 + (Mnvp2/Vs) · (0.5 – χ) c + (Mnvp3/Vs) c2/3 + …} Π / c ≈ RT · {Mn1 + A2 c + A3 c2 + …} According to this equation, the osmotic pressure of an ideal polymer solution rapidly decreases with increasing molecular weight, i.e., the driving force for polymer-polymer mixing is rather small.

## How is molecular weight determined?

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Sample Molecular Weight Calculation

Using the periodic table of the elements to find atomic weights, we find that hydrogen has an atomic weight of 1, and oxygen’s is 16. In order to calculate the molecular weight of one water molecule, we add the contributions from each atom; that is, 2(1) + 1(16) = 18 grams/mole.

What is the most accurate method to determine molecular weight?

Osmotic pressure method is best for the determination of molecular weight of polymers and protiens since osmotic pressure though very small, is measurable.

What does molecular weight depend on?

The weight average molecular weight depends not only on the number of molecules present, but also on the weight of each molecule.

How is the molecular weight of a polymer determined?

Determining Polymer Molecular Weight

Molecular weight is determined by multiplying the mass of each subsequent repeating unit in the polymer chain, before adding the mass of either end group. This provides a range of values that are used to establish the molecular weight distribution of the material.

References:

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